Geological Survey

Description

US Geological Survey, National Wildlife Heath Center, is offering a funding opportunity to a CESU Partner for research to characterize the limits of sensitivity of two emerging, low-cost NGS platforms. Oxford Nanopore¿s technology promises rapid time to pathogen identification as the platform produces data for analysis in real time, rather than having to wait hours or days required to complete a NGS run using a more conventional platform. Will characterize the sensitivity of the Oxford Nanopore Minion platform for the identification of a viral pathogen directly from a sample in as little as 30 minutes. Will also characterize the minimal amount of virus necessary in a sample for the assembly of a whole viral genome using the Illumina iSeq 100 system. The iSeq platform provides an ideal combination of high data density inherent in Illiumina technology but downscaled for single, high-value samples. Finally, will standardize the procedures we develop for the two platforms into SOPs, improve the analytical software for analysis on both platforms, and disseminate this information so that other NAHLN labs can quickly adopt the capability for rapid detection of emerging pathogens.

Who can apply

• Others

Contact

Faith Graves 703-648-7356 <br/>fgraves@usgs.gov
fgraves@usgs.gov